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The Impact of Customized Surface Topography and Porosity Created by Additive Manufacturing Technology

Now online in the Journal of Prosthodontics, a research article co-authored by ACP members Franciele Floriani, DDS, MS, PhD and Amirali Zandinejad, DDS, MSc.

This research analyzed gingival fibroblast proliferation on additively manufactured polymethylmethacrylate (PMMA) groups with different surface characteristics (a no treatment group and a customized 250 µm diameter porosity group). For each group 20 samples of material were tested. Rat gingival fibroblasts were used to investigate the soft tissue attachment of the materials in this study. The fibroblast cells, at a concentration of 2.5 × 104 cells/mL, were seeded into 40 plates with the sterile no treatment and Porous PMMA plates separately.

For cell proliferation MTT assay was conducted at 24, 48, and 72 hours. Cell proliferation was quantified through optical density (OD) measurements of the cell supernatant, and surface analysis was conducted using a scanning electron microscope (SEM).

At 24 hours, the no treatment group surface demonstrated superior cell proliferation compared to the surface with porosity. However, significant differences in cell growth on both materials at 48 and 72 hours, suggesting that both surface types eventually support similar levels of cell proliferation.

Khurana S, Floriani F, Li Y, Liu X, Zandinejad A. The impact of customized surface topography and porosity created by additive manufacturing technology on gingival fibroblasts. J Prosthodont. 2025; 1–6. https://doi.org/10.1111/jopr.14030

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